International Journal of Dentistry Research 2025; 10(3): 102-109 DOI:10.31254/dentistry.2025.10306

Research Article

Comparative Evaluation of Anesthetic and Antimicrobial efficacy of 10% Propolis hydrogel with Scaling and Root planing in Generalized Chronic Periodontitis - A Clinico-Microbiological, Split Mouth Study

Manav Varshney¹ , Akash A Bora¹ , Rahul Chopra² , Sumit Malhotra³

Manav Varshney,Akash A Bora,Rahul Chopra,Sumit Malhotra

1. Post Graduate, Department of Periodontology and Oral Implantology, ITS Centre for Dental Studies and Research, Ghaziabad -201206, Uttar Pradesh, India
2. Professor, Department of Periodontology and Oral Implantology, ITS Centre for Dental Studies and Research, Ghaziabad - 201206, Uttar Pradesh, India
3. Professor and Head, Department of Periodontology and Oral, Implantology, ITS Centre for Dental Studies and Research, Ghaziabad-201206, Uttar Pradesh, India

*Author to whom correspondence should be addressed.

Received: 28th November, 2025 / Revised: 1st January, 1970 / Accepted: 8th January, 2025 / Published : 17th January, 2026

Abstract

Background: Chronic periodontitis is a common inflammatory disease leading to the destruction of periodontal tissues. While scaling and root planing (SRP) remains the cornerstone of treatment, adjunctive agents can enhance therapeutic outcomes. Propolis, a natural bioactive compound, possesses significant antimicrobial and anesthetic properties. This split-mouth study was conducted to evaluate the efficacy of 10% Propolis hydrogel as an adjunct to SRP in improving  clinical parameters and reducing microbial load in generalized chronic periodontitis. Methodology: 15 patients with generalized chronic periodontitis having pocket depth ≥ 4mm were selected. Maxillary and mandibular arch of each patient was randomly divided to receive either of Placebo gel (Group A) or 10% Propolis Hydrogel (Group B) followed by SRP and application of gel in periodontal pockets. Clinical parameters, VAS score were assessed and quantitative analysis of sub-gingival plaque samples for aerobic and anaerobic bacterial growths using colony count method at baseline and after 3 months was performed. Results: Intragroup comparison shows statistically significant results in all the clinical and microbiological parameters. Intergroup comparison showed statistically significant amount of reduction in pockets and Colony Forming Unit (CFU/ml) of aerobic as well as anaerobic bacteria in Group B when compared to Group A. Significant results were also found in Group B in terms of VAS score (assessing dentinal sensitivity) in both the groups. Conclusion: The adjunctive use of 10% propolis hydrogel with SRP provides enhanced anesthetic and antimicrobial benefits in the treatment of generalized chronic periodontitis, suggesting its potential as a valuable adjunct to mechanical therapy.